Year: 2014 | Month: September | Volume 7 | Issue 3
In vitro Plant Regeneration from Seedlings-derived Explants of Tomato (Lycopersicon esculentum Mill.)
Abstract:
Tomato is one of the most important vegetable crops in India. In vitro tissue culture of tomato could serve as an important means for its improvement through genetic transformation. To improve the regeneration capacity of tomato, the effect on plant regeneration of donor plant type, basal medium, and plant growth regulators were evaluated using explants derived from the cotyledon, hypocotyl, leaf and petioles. Cotyledon and hypocotyl excised from 10-15 days old and leaf and petiole excised from 25-30 days were optimal explants. Explants were cultured on MS (Murashige and Skoog) basal medium supplemented with different concentrations and combinations of BA-IAA, BA-NAA, Kn-IAA and Kn-NAA. The highest regeneration efficiency was obtained on MS basal medium containing combinations of BA-IAA and Kn-IAA. The best response in terms of the percentage of shoot regeneration (77%) was obtained from petiole explant cultured on MS basal medium supplemented with 1.0 mg/l Kn + 1.0 mg/l IAA. Successful rooting was achieved by placing the shoots onto MS basal medium supplemented with 0.20 mg/l NAA. The combination of sand: soil (1:1) was the best for plant acclimatization as 90% of the plants survived and became established.
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